r d systems af2557  (R&D Systems)

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Massive accumulation of luminal protease-deficient axonal lysosomes at Alzheimer’s disease amyloid plaques

doi: 10.1073/pnas.1510329112

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: Additional tissue-staining reagents included Thioflavin S (Sigma-Aldrich) and BODIPY-FL-Pepstatin A (Life Technologies). table ft1 table-wrap mode="anchored" t5 Table S1. caption a7 Antibody Source Catalog/clone no. Dilution LAMP1 DSHB 1D4B 1:250 EEA1 Thermo ABR PAI-063A 1:100 Dynamin 1 Epitomics 1851–1 1:100 BIN1 Millipore 5–449 1:100 vGLUT1 Millipore AB5905 1:1,000 M6PR DSHB 86f7 1: 400 Iba-1 Wako 019–19741 1:250 Legumain/AEP R&D Systems AF2058 1:200 RagC Cell Signaling Technology D8H5 1:100 PGRN R&D Systems AF2557 1:200 Cathepsin B R&D Systems AF965 1:200 Cathepsin L R&D Systems AF1515 1:200 Amyloid-β Cell Signaling Technology 2454 1:250 PDI Stressgen SPA-891 1:100 Rab5 Cell Signaling Technology C8B1 1:100 VAMP2 Synaptic Systems C1 69.1 1:200 Cytochrome C BD Biosciences 556432 1:100 CD-68 AbD Serotec MCA 1957T 1:250 GFAP Dako Z0334 1:200 Map2B BD Biosciences 610410 1:100 Neurofilament Covance SMI311 1:200 Grasp-65 Abcam Ab30315 1:3,000 BACE-1 Cell Signaling Technology 5606 1:100 SORLA Abcam Ab16642 1:100 Sortilin 1 Abcam Ab16640 1:100 Open in a separate window Antibodies used in the study

Techniques: